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Ultrapure Total RNA Extraction Kit Through the perfect combination of extraction technology and column purification technology, the entire process of RNA isolation and purification with this kit can be completed within 20-40 minutes.Compared with the classic Trizol precipitation method, the total RNA obtained by this kit has lower salt and protein residues, and can be directly used for Northern blot analysis, dot hybridization, poly(A)+ selection, in vitro translation, RNase protection analysis and other experiments. .Ultrapure Total RNA Extracti Kit Cat. No. | 5 preps 5003005 | 5pres 5003050 |
Grinding rods Spin columns Buffer TL Buffer EX Buffer DW (Concentrate) Buffer WA (concentrate) Buffer WBR (concentrate) RNase-free Water Manual | 5 sticks 5 sets 6ml 1.2ml 1 ml 1.9 ml 1.5 ml 1.5ml 1 copy | 50 sticks 50 sets 55ml 12ml 10 ml 12 ml 10 ml 2ml×3 1 copy |
Buffer TL can be transported at room temperature, please store at 2~8 ℃ after receiving the product.
If other items and reagents are stored at normal temperature (0~30°C), their performance will remain unchanged within three years; if the product is stored at 2~8°C, the validity period of the product can be extended to more than three years.
Buffer TL is a ready-to-use reagent for total RNA extraction from cells and tissues. In homogenized or lysed samples, Buffer TL lyses cells and releases RNA while maintaining RNA integrity. After adding Buffer EX for extraction and centrifugation, the cell lysate is separated into an aqueous phase and an organic phase, and RNA is present in the aqueous phase. Add Buffer DW to the aqueous phase containing RNA, then add the mixture to the Spin column for centrifugation, the RNA is adsorbed to the Spin column. After washing with Buffer WA and Buffer WBR to remove residual proteins and PCR inhibitors, elute the RNA with RNase-free Water. The eluted RNA can be immediately used in various molecular biology experiments.
Through the perfect combination of extraction technology and column purification technology, the entire process of RNA isolation and purification with this kit can be completed within 20-40 minutes. Compared with the classic Trizol precipitation method, the total RNA obtained by this kit has lower salt and protein residues, and can be directly used for Northern blot analysis, dot hybridization, poly(A)+ selection, in vitro translation, RNase protection analysis and other experiments. .
Equipment and Reagents to Be Supplied by User
1. Absolute ethanol
2. 1.5 ml centrifuge tube (RNase-free 1.5 ml centrifuge tube must be used)
3. Pipettes and tips (to avoid RNase contamination, please use RNase-free pipette tips with filters)
4. Latex gloves, disposable masks and other protective equipment and paper towels
5. Desktop centrifuge (can be equipped with a rotor for centrifuging 1.5 ml centrifuge tubes and 2 ml collection tubes)
6. Vortexer
7. RNase-free RNA extraction laboratory
8. Liquid nitrogen and mortar, RNA Later (Cat. No. 4007020), and DNase I on-column digestion kit (Cat. No. 8010050) may be required.
Buffer TL is a ready-to-use reagent for total RNA extraction from cells and tissues. In homogenized or lysed samples, Buffer TL lyses cells and releases RNA while maintaining RNA integrity. After adding Buffer EX for extraction and centrifugation, the cell lysate is separated into an aqueous phase and an organic phase, and RNA is present in the aqueous phase. Add Buffer DW to the aqueous phase containing RNA, then add the mixture to the Spin column for centrifugation, the RNA is adsorbed to the Spin column. After washing with Buffer WA and Buffer WBR to remove residual proteins and PCR inhibitors, elute the RNA with RNase-free Water. The eluted RNA can be immediately used in various molecular biology experiments.
Through the perfect combination of extraction technology and column purification technology, the entire process of RNA isolation and purification with this kit can be completed within 20-40 minutes. Compared with the classic Trizol precipitation method, the total RNA obtained by this kit has lower salt and protein residues, and can be directly used for Northern blot analysis, dot hybridization, poly(A)+ selection, in vitro translation, RNase protection analysis and other experiments. .
This product can be perfectly used for the extration of total RNA from tissues and cells of various human, animal, plant or bacterial origins. The applicable starting sample sizes are as follows:
sample | Recommended dosage |
Animal tissues (liver, brain and other easily homogenized tissues) | 10~100 mg |
Animal tissues (skin, bones, etc.) | 100~200 mg |
Blood (erythrocytic nucleated organisms) | 200 µl |
Blood (erythrocytic anucleate) | 300~500 µl |
Cultured cells | 5~10×10 6 |
bacteria | 50~100 mg |
* Plant tissue | 50~100 mg |
* Some plant tissues have high polysaccharide content are not suitable for this product. It is recommended to use the High Polysaccharide and Polyphenol Plant Total RNA Kit (Cat. No. 5103050) to extract RNA.
1. If the centrifuge has a refrigeration function, please set the temperature to 25°C.
2. Add absolute ethanol to Buffer DW, Buffer WA and Buffer WBR according to the instructions on the label of the reagent bottle, and check the box on the label to mark "Ethanol has been added".
3. Because saliva and skin contain RNase, latex gloves and masks are required throughout the RNA extraction process.
