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Whole Blood Total RNA Extraction Kit is a rapid silica-column based purification system designed for the isolation of high-quality total RNA directly from whole blood samples.
Unlike conventional methods requiring red blood cell lysis and leukocyte separation, this kit enables direct RNA extraction from whole blood, significantly reducing sample handling time and minimizing RNA degradation.
Most importantly, the kit has been experimentally validated for RNA extraction from anticoagulated whole blood stored at -80°C for 75 days, making it highly suitable for:
Biobanking samples
Retrospective clinical studies
Molecular diagnostics
Gene expression analysis
RT-PCR and RNA sequencing
Validated for whole blood samples stored at -80°C for up to 75 days.
High-quality RNA can be recovered directly from frozen anticoagulated whole blood without prior leukocyte isolation.
Traditional blood RNA extraction workflows require:
Red blood cell lysis
Leukocyte separation
Multiple centrifugation steps
Our kit uses a direct lysis strategy, eliminating these labor-intensive procedures.
The kit is suitable for:
Whole blood
Frozen blood
Bone marrow aspirates
making it a versatile solution for hematology and oncology research.
Purified RNA demonstrates:
High purity
Excellent integrity
Strong RT-qPCR performance
RNA sequencing compatibility
Ideal for downstream applications including:
RT-PCR
RT-qPCR
RNA-Seq
Gene expression profiling
RNA purification can be completed in only:
20–40 minutes
compared with:
60–120 minutes for traditional leukocyte-based methods.
Optimized for Challenging Samples (-80°C Frozen Blood)Extracting high-quality RNA from frozen blood samples is notoriously difficult due to rapid RNase activation upon thawing. Our kit is specifically designed to handle this challenge. For a step-by-step optimization protocol, check out our laboratory guide on
Experimental results demonstrated:
· Successful RNA purification
· High RNA purity
· Excellent electrophoresis performance
· Positive RT-qPCR amplification
· Reliable downstream application compatibility
These results confirm the suitability of the kit for long-term frozen blood storage applications.
Figure Validation Overview:
Total RNA successfully isolated from anticoagulated whole blood samples stored at -80°C for 75 days using our Whole Blood Total RNA Extraction Kit.
RNA Integrity (Left): Agarose gel electrophoresis demonstrates sharp, clear 28S and 18S rRNA bands with minimal degradation, confirming exceptional RNA purity and integrity.
Downstream Compatibility (Right): Strong and consistent RT-qPCR amplification curves ($\beta$-actin) confirm that the extracted RNA is free of inhibitors and fully compatible with sensitive downstream molecular applications.
To evaluate long-term frozen blood compatibility, anticoagulated whole blood samples were stored at -80°C for 75 days.
Proven Direct Extraction Method as demonstrated above, our optimized protocol allows for direct isolation from frozen samples without complex pre-thawing steps, preserving maximum RNA integrity. Read the complete workflow details in our
| Feature | Traditional Methods | Whole Blood Total RNA Extraction Kit |
|---|---|---|
| RNA Extraction from -80°C Frozen Blood | Limited | ✔ Yes |
| Long-Term Frozen Blood Validation | Rarely Reported | ✔ Validated (75 Days) |
| RBC Lysis Required | Yes | No |
| Leukocyte Separation | Yes | No |
| Processing Time | 60–120 min | 20–40 min |
| Bone Marrow Compatible | Limited | ✔ Yes |
| RT-PCR Ready | Yes | ✔ Yes |
| RNA Sequencing Compatible | Yes | ✔ Yes |
Gene expression analysis
Biomarker discovery
Transcriptomics
Frozen biobank samples
Longitudinal studies
Clinical specimen analysis
RT-PCR
RT-qPCR
RNA Sequencing
cDNA synthesis
NGS library preparation
Blood Total RNA Kit Cat. No. | 5 preps FR5201005 | 50 preps FR5201050 |
Spin Columns 2 ml Collection Tubes Buffer L9 Buffer WA (concentrate) Buffer WBR (concentrate) RNase-free Water Instructions | 5 5 6 ml 1.9 ml 1.5 ml 1.5 ml 1 copy | 50 50 55 ml 12 ml 12 ml 2 ml×2 1 copy |
1. Buffer L9 can be transported at room temperature, please store at 2~8 ℃ after receiving the product.
2. Other reagents and components are stored at room temperature (0~30 ℃), the performance of the product can be maintained without significant change within two years; if the product is stored at 2~8 ℃, the validity period of the product can be extended to more than two years (the product stored at 2~8℃ should be restored to room temperature before use).
Yes. Our kit is specifically validated for whole blood stored at -80°C (e.g., up to 75 days). It effectively neutralizes RNases immediately upon contact with the sample, preventing degradation.
For optimal RNA integrity, we recommend following our direct extraction protocol which minimizes thawing time to prevent RNA degradation.
No. Direct RNA extraction from whole blood is supported.
No. The workflow eliminates the need for leukocyte isolation.
Yes. The kit is compatible with bone marrow specimens.
RT-PCR, RT-qPCR, RNA-Seq, transcriptomics, viral RNA detection, fusion gene analysis, and gene expression profiling.
Approximately 20–40 minutes.
Yes. The purified RNA is fully compatible with RNA-Seq workflows.
Unlike most commercial blood RNA extraction kits, this product has been experimentally validated for direct RNA extraction from anticoagulated whole blood stored at -80°C for 75 days, while maintaining excellent RT-qPCR performance and RNA quality.
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Direct RNA Extraction from -80°C Frozen Whole Blood or 500uL fresh blood
