ETV6 Dual Color Break Apart Probe Fluorescence in situ hybridization (FISH) Reagents

 Intended use

ETV6 Dual Color Break Apart Probe  Fluorescence in situ hybridization (FISH) Reagents is used to qualitatively detect the abnormality of human ETV6（12p13）gene in cytological samples or paraffin-embedded tissue samples in vitro. This kit is not used for clinical evaluation in combination with specific drugs, and its test results are for clinical reference only, and should not be used as the sole basis for individualized treatment of patients. Clinicians should comprehensively judge the test results according to the patient's condition, drug indications, treatment response and other laboratory indicators.

 Test principle

According to the DNA base complementary pairing principle, the ETV6 orange-red probe and the ETV6 green probe were used to hybridize with the DNA target sequence in the nucleus, and the gene state information in the nucleus was observed and analyzed under a fluorescence microscope.

Kits Component

Tissue specimen

The paraffin-embedded tissue specimens were fixed in 10% neutral buffered formalin, and the slice thickness was between 3 and 5 µm.

Cell specimen

The fresh samples of bone marrow and peripheral blood were preserved at 4 ℃ for no more than 24 hours, and the fixed cell suspension was preserved at-20 ℃.

Storage Condition and Expiration

Stored at -20℃±5℃ in the dark, valid for 12 months. Transport below 8 ℃.