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HER2/CSP17 Dual Color Probe Fluorescence in situ hybridization (FISH) Reagents

  • highlight iconHER2/CSP17 Dual Color Probe Fluorescence in situ hybridization (FISH) Reagentsis used to qualitatively detect the amplification of HER2 gene in human breast cancer tissue sections fixed with 10% neutral buffered formalin and embedded in paraffin.
  • highlight iconFluorescence in situ hybridization (FISH) is based on the principle of complementary pairing of DNA bases, and the visualization of hybridization signals of fluorescence-labeled DNA probes with its DNA target sequences in cell nucleus under fluorescence microscope.



Intended use

HER2/CSP17 Dual Color Probe  Fluorescence in situ hybridization (FISH) Reagentsis used to qualitatively detect the amplification of HER2 gene in human breast cancer tissue sections fixed with 10% neutral buffered formalin and embedded in paraffin.

 

Test principle

Fluorescence in situ hybridization (FISH) is based on the principle of complementary pairing of DNA bases, and the visualization of hybridization signals of fluorescence-labeled DNA probes with its DNA target sequences in cell nucleus under fluorescence microscope.high-quality lens oil.

 

Kit Component



 

Component             Specifications    Main   Ingredient
5 Tests10 Tests20 Tests
HER2/CSP17
DualColorProbe
50μl100μl200μl   HER2 orange probe,
    CSP17 green probe,
    hybridization buffer


 

Specimen requirements

The specimens were paraffin-embedded breast cancer tissue sections confirmed by pathological examination.

In the preparation of paraffin sections, the fresh tissue should be fixed in 10% neutral buffer formalin fixed solution for 6 hours within 1 hour; the thickness of the section should be between 3μm and 5 μm; the wax blocks or slices can be kept stable for 12 months after drying at room temperature.

 

 

Storage Condition and Expiration

Stored at -20±5 in the dark, valid for 12 months.


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